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Figure 6 | Respiratory Research

Figure 6

From: Respiratory epithelial cells require Toll-like receptor 4 for induction of Human β-defensin 2 by Lipopolysaccharide

Figure 6

ΔMyD88 and ΔMal inhibit LPS-induced HBD2 expression in A549 cells. A549 cells (1.5 × 105) were transfected with pCDNA3.1 or pDC304 (empty vectors), ΔMyD88 or Mal P/H expression plasmids as indicated. 24 h post transfection, cells were stimulated with LPS (10 μg/ml) for 24 h. HBD2 expression was measured by semi-quantitative RTPCR. Expression in LPS-treated cells was ascribed a value of 100 %. Data shown are mean+/- S.E. (n = 3). Regarding amounts of transfected DNA, ΔMyD88 + and ++ represent 100 and 200 ng of dominant negative MyD88 plasmid DNA respectively while Mal + and ++ represent 50 and 100 ng of dominant negative Mal plasmid DNA respectively. (* P < 0.05, ** P < 0.01, *** P < 0.005 vs control + LPS)

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