Effects of nano particles on antigen-related airway inflammation in mice

Respiratory Research

Table 5 Levels of total IgE and antigen-specific IgG1, IgG2a, and IgE.

Group	Animals (n)	Total IgE (ng/ml)	Antigen-Specific IgG₁ (titers)	Antigen-Specific IgG_2a (titers)	Antigen-Specific IgE (fluorescene intensity)
vehicle	12	93.8 ± 27.5	485.3 ± 274.2	152.1 ± 48.8	625.2 ± 49.3
14 nm	9	152.3 ± 36.1	1880.3 ± 1656.2	163.5 ± 49.2	698.7 ± 61.0
56 nm	11	304.1 ± 127.4	1128.2 ± 717.1	182.5 ± 72.9	610.9 ± 54.1
OVA	10	443.4 ± 173.2	2178.5 ± 910.7	256.7 ± 206.2	576.2 ± 89.3
OVA + 14 nm	10	871.9 ± 246.7** ^{# $}	28050.3 ± 13840.4** ^{## $}	354.9 ± 141.7	860.7 ± 120.5* ^##
OVA + 56 nm	10	515.6 ± 122.4*	4150.5 ± 1657.0	126.7 ± 32.2	600.7 ± 62.8

Six groups were intratracheally inoculated with vehicle, nano particles, OVA, or the combination of OVA and nano particles for 6 wk. Serum samples were retrieved 24 h after the last intratracheal instillation. Total IgE and antigen-specific IgG₁, IgG_2a, and IgE were analyzed using ELISA. Results are expressed as mean ± SEM. *P < 0.05 versus vehicle, **P < 0.01 versus vehicle, ^#P < 0.05 versus OVA, ^##P < 0.01 versus OVA, ^$P < 0.01 versus nano particles.

ISSN: 1465-993X