Effects of nano particles on antigen-related airway inflammation in mice

Respiratory Research

Table 4 Protein levels of eotaxin, MCP-1, RANTES, and IL-6 in the lung tissuesupernatants.

Group	Animals	eotaxin	MCP-1	RANTES	IL-6
	(n)	(pg/total lung tissue supernatants)
vehicle	16	67.9 ± 2.9	20.8 ± 3.5	174.6 ± 15.8	105.8 ± 3.6
14 nm	13	99.4 ± 7.1	239.0 ± 17.4**	192.3 ± 18.0	151.7 ± 8.2
56 nm	14	89.5 ± 9.2	89.3 ± 7.4	201.6 ± 22.1	106.5 ± 4.9
OVA	16	130.9 ± 32.3	41.9 ± 9.2	160.7 ± 17.1	118.3 ± 5.7
OVA + 14 nm	16	804.8 ± 175.7** ^{## $$}	542.8 ± 45.4** ^{## $$}	432.6 ± 27.8** ^{## $$}	297.7 ± 30.2** ^{## $$}
OVA + 56 nm	17	399.1 ± 86.9** ^{# $}	124.0 ± 14.1** ^#	235.4 ± 16.8* ^#	202.4 ± 54.2** ^{# $$}

Six groups were intratracheally inoculated with vehicle, nano particles, OVA, or the combination of OVA and nano particles for 6 wk. Lungs were removed and frozen 24 h after the last intratracheal administration. Protein levels in the lung tissue supernatants were analyzed using ELISA. Results are shown as mean ± SEM. *P < 0.05 versus vehicle, **P < 0.01 versus vehicle, ^#P < 0.05 versus OVA, ^##P < 0.01 versus OVA. ^$P < 0.05 versus nano particles. ^$$P < 0.01 versus nano particles.

ISSN: 1465-993X