Effects of nano particles on antigen-related airway inflammation in mice

Table 1 Cellular profile in bronchoalveolar lavage fluid.

Group	Animals (n)	Total Cells (× 10 ⁴ /total BAL)	Macrophages (× 10 ⁴ /total BAL)	Eosinophils (× 10 ⁴ /total BAL)	Neutrophils (× 10 ⁴ /total BAL)	Mononuclear Cells (× 10 ⁴ /total BAL)
vehicle	16	36.88 ± 3.56	36.74 ± 3.53	0 ± 0	0.12 ± 0.05	0.015 ± 0.01
14 nm	13	111.69 ± 9.27**	83.79 ± 6.03**	0.332 ± 0.176	27.04 ± 4.98**	0.491 ± 0.201*
56 nm	14	97.36 ± 16.06**	88.64 ± 15.34**	0.331 ± 0.177	8.09 ± 2.49*	0.265 ± 0.093
OVA	16	85.06 ± 12.63**	81.91 ± 12.4**	0.705 ± 0.255	2.2 ± 0.62	0.121 ± 0.059
OVA + 14 nm	16	193.69 ± 18.33** ^{## $$}	141.86 ± 14.97** ^{## $}	13.667 ± 4.731** ^{## $}	36.9 ± 3.67** ^{## $}	0.878 ± 0.232** ^#
OVA + 56 nm	17	102.65 ± 11.64**	90.7 ± 10.12**	3.984 ± 2.669	7.79 ± 2.29*	0.204 ± 0.073

Six groups of mice were intratracheally administered with vehicle, ovalbumin (OVA), nano particles, or combinations of OVA and nano particles for 6 wk. Bronchoalveolar lavage (BAL) was conducted 24 h after the last intratracheal instillation. Total cell counts were determined on fresh BAL fluid, and differential cell counts were assessed with Diff-Quik-staining. Results are presented as mean ± SEM. *P < 0.05 versus vehicle, **P < 0.01 versus vehicle, ^#P < 0.05 versus OVA, ^##P < 0.01 versus OVA. ^$P < 0.05 versus nano particles. ^$$P < 0.01 versus nano particles.