Figure 6

Inhibition of HCV-core induced IL-8 expression by antibody to gC1qR. A. gC1qR expression in NHLF. NHLF were mock-treated (1) or treated with β-galactosidase (2), β-galactosidase-HCV core protein (3), or β-galactosidase-HCV core protein and TNFα (4) and incubated for 24 h. Whole cell lysates of NHLF were subjected to SDS-PAGE, immunoblotted with antibodies specific for gC1qR, and visualized using ECL. A 33 kD protein was identified. B. Inhibition of HCV core-induced IL-8 expression by gC1qR. NHLF were mock-treated (Mock) or treated with β-galactosidase (β-gal), β-galactosidase-HCV core protein (Core), or β-galactosidase-HCV core protein with either antibody to gC1qR at the indicated concentrations or mouse IgG at 1 μg/ml. Supernatants were collected at 24 h and IL-8 expression assayed by ELISA as described in Materials and Methods. All assays were done in triplicate.