Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells

Respiratory Research

Table 8 Apoptotic parameters upon sublethal hyperoxia of rats followed by normoxia

	Hyperoxia followed by normoxia for
	48 hrs hyperoxia	24 hrs normoxia	48 hrs normoxia
Caspase 3 activity	171 ± 18*	97 ± 16	73 ± 29
Early apoptotic cells	102 ± 13	95 ± 14	109 ± 26
Late apoptotic cells	106 ± 29	125 ± 25	85 ± 17
Cell death detection ELISA	97 ± 23	104 ± 6	95 ± 14

Activity of caspase 3 and parameters of apoptosis detected in freshly prepared TIIcells of rats exposed to oxygen for 48 hrs (hyperoxia) followed by normoxia for 24 or 48 hrs as described in Materials and Methods. Values are means ± S.D. given in arbitrary units (normoxic control = 100) of n = 3 independent experiments. Asterisk indicates a significant difference compared to control (p < 0.05)

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