Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells

Respiratory Research

Table 2 Effect of hyperoxia on the TNFα content in different specimen from rat

	Control	Hyperoxia
Plasma (pg/ml)	106 ± 31	149 ± 11
Macrophages (ng/mg protein)	11.4 ± 3.7	19.4 ± 2.8*
TIIcells (ng/mg protein; n = 6)	18.5 ± 2.1	27.2 ± 6.7*
Bronchoalveolar lavage (pg/ml)	109 ± 1	172 ± 38*
Spontaneous secretion of TNFα by TIIcells (ng × mg cell protein^-1 × hr^-1)	6.3 ± 1.3	21.2 ± 7.5*

Concentration of TNFα was determined in plasma, macrophages, TIIcells and bronchoalveolar lavage of rats exposed to air (control) or oxygen for 48 hrs (hyperoxia) by ELISA as described in Materials and Methods. Spontaneous secretion of TNFα was measured in cell-free supernatant upon incubation of freshly isolated TIIcells in DMEM for 30 min at 37°C. TNFα concentrations are given as means with standard deviation of n = 3 independent experiments unless stated otherwise. Asterisk indicates a significant difference to control (p < 0.05).

ISSN: 1465-993X