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Table 2 nAChR Antibodies and Conditions

From: Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

Subunit

Company

Dilution

alpha-1

Sigma

1:30,000

alpha-2

Santa Cruz

1:1000

alpha-3

Santa Cruz

1:2000

alpha-4

Santa Cruz

1:1000

alpha-5

Sigma

1:15,000

alpha-7

Sigma

1:30,000

beta-1

Sigma

1:10,000

beta-2

Santa Cruz

1:1,000

delta

Wang et al. [30]

1:4000

  1. Antibodies specific to particular nAChR subunits were purchased from either Santa Cruz or Sigma. Antibody specific to the δ subunit was a gift from Dr. Zhu-Zhong Wang, University of Pittsburgh.