Nicotine signals through muscle-type and neuronal nicotinic acetylcholine receptors in both human bronchial epithelial cells and airway fibroblasts

Table 1 RT-PCR Primers and Conditions

Primer	Optimal Annealing Temperature	Product Size	Sequence
alpha 1*	55	580/505	CGT TCT GGT GGC AAA GCT
			CCG CTC TCC ATG AAG TT
alpha 2*	55	466	CCG GTG GCT TCT GAT GA
			CAG ATC ATT CCA GCT AGG
alpha 3	58	464	CTG GTG AAG GTG GAT GAA GT
			CTC GCA GCA GTT GTA CTT GA
alpha 4	58	444	GGA TGA GAA GAA CCA GAT GA
			CTC GTA CTT CCT GGT GTT GT
alpha 5*	55	525	GAT AAT GCA GAT GGA CGT
			TGA TGG TAT GAT CTC TTC
alpha 6	58	372	GTG GCC TCT GGA CAA GAC AA
			CCT GCA GTT CCA AAT ACA CA
alpha 7	58	375	GGA GCT GGT CAA GAA CTA CA
			CAG CGT ACA TCG ATG TAG CA
beta 1	58	479	CTA CGA CAG CTC GGA GGT CA
			GCA GGT TGA GAA CCA CGA CA
beta 2	62	453	CAA TGG CTC TGA GCT GGT GA
			CCA CTA GGT GTG AAG TCG TCC A
		420	GGC TCT GAG CTG GTG ACA GTA
			CAC CTC ACT CTT CAG CAC CA
beta 3	62	439	TGGAGA GTA CCT GCT GTT CA
			CGA GCC TGT TAC TGA CAC TA
beta 4	58	524	GTG AAT GAG CGA GAG CAG AT
			GGG ATG ATG AGG TTG ATG GT
delta	58	471	CAG ATC TCC TAC TCC TGC AA
			CCA CTG ATG TCT TCT CAC CA
		426	CAA CGT GCT TGT CTA CCA CTA C
			GGT AGG TAG AAG ACC AGG TTG A
gamma		546	CGC CTG CTC TAT CTC AGT CA
			GGA GAC ATT GAG CAC AAC CA
epsilon	55	432	GTA ACC CTG ACG AAT CTC AT
			GTC GAT GTC GAT CTT GTT GA

* From Maus et al. [12]
Unique primers to each nAChR subunit were used in PCR reactions. All results were sequenced and compared to the known subunit sequences to confirm that the correct subunit was being amplified.

ISSN: 1465-993X