Table 5 Studies utilizing microarray technology to study ALI/PE

Number of genes

McDowell et al.⁵⁶

cDNA 8.374 genes

6 mice Lung tissue samples

Differential gene expression in the initiation and progression of nickel-induced ALI.

Ratios of Cy5/Cy3 multiplied to the balance coefficient of each microarray / 5 replicates

Olman et al.⁵⁸

cDNA 588 genes

36 patients Pulmonary edema Lung fibroblasts

Microarray analysis indicates that pulmonary edema fluid from patients with ALI mediates inflammation, mitogen gene expression, and fibroblast proliferation through bioactive IL-1.

G.I normalized to the housekeeping G.I 2 replicates

Kupfner et al.⁶¹

Oligonucleotide

Mice Lung neutrophils

Role of NF-κB in endotoxemia-induced alterations of lung neutrophil apoptosis.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 3 replicates

Cher et al.⁶²

Oligonucleotide 8.800 genes

21 rats Whole lungs

Pulmonary inflammation and edema induced by Phospholipase A_2.

Each gene was divided by the median of its values in all samples / 3 replicates

Sabbadini et al.⁶³

Oligonucleotide 12.600 genes

14 rabbits Lung tissue samples

Gene expression analysis in interstitial lung edema induced by saline infusion.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 2 replicates

Perkowski et al.⁶⁴

cDNA 8.374 genes

20 mice Lung tissue samples

Gene expression profiling of the early pulmonary response to hyperoxia in mice.

Difference between observed log-ratio and corresponding fitted ratio/ 5 replicates

Ward et al.⁶⁵

cDNA 7.398 genes

6 rats Lung and other organ samples

Molecular signatures of sepsis: multiorgan gene expression profiles of systemic inflammation.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 4 replicates