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Table 4 Studies utilizing microarray technology to study COPD

From: Application of microarray technology in pulmonary diseases

Investigator

Microarray type

Species/Sample size

Summary/Key findings

Normalization procedure

 

Number of genes

Type of tissue

 

Replications per data point

Koike et al.45

cDNA 450 genes

Rats AM

cDNA microarray analysis of gene expression in rat alveolar macrophages in response to organic extract of diesel exhausts particles.

G.I was normalized to the housekeeping G.I 2 replicates

Yamanaka et al.46

cDNA 18.432 genes

Human AEC

Gene expression profiles of human small airway epithelial cells treated with low doses of 14- and 16-membered macrolides.

G.I was normalized to the housekeeping G.I 3 replicates

Fuke et al.47

cDNA 77 genes

30 patients Lung tissue specimens

Chemokines in bronchiolar epithelium in the development of chronic obstructive pulmonary disease.

Signal normalized to a given gene transcript 3 replicates

Vuillemenot et al.48

Oligonucleotide 12.000 genes

10 mice Lung tissue specimens

Lymphoid tissue and emphysema in the lungs of transgenic mice inducibly expressing tumor necrosis factor-alpha.

Signal normalized to internal control 2 replicates

Hackett et al.50

cDNA 4.600 genes

22 individuals AEC

Variability of antioxidant-related gene expression in the airway epithelium of cigarette smokers.

Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level / 2 replicates

Morris et al.52

Oligonucleotide 6.500 genes

Mice Lung tissue samples

Loss of integrin alpha (v) beta6-mediated TGF-beta activation causes MMP-12-dependent emphysema.

Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level / 2 replicates

Golpon et al.53

Oligonucleotide 6.500 genes

Human/mice Lung tissue samples

HOX genes in human lung: altered expression in primary pulmonary hypertension and emphysema.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs/ 3 replicates

  1. Abbreviations: AEC: Airway Epithelial Cells, AM: Alveolar Macrophages, COPD: Chronic Obstructive Pulmonary Disease, G.I: Gene Intensity, TGF-b: Transforming Growth Factor-beta, MMP: Metalloproteinase