Table 3 Studies utilizing oligonucleotide microarray technology to study asthma

Number of genes

Lee et al.³⁴

Oligonucleotide 6.500 genes

Human airway cells

IL-13 induces dramatically different transcriptional programs in three human airway cell types.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched)/ 1 replicate

Temple et al.³⁵

Oligonucleotide 6800 genes

Human eosinophils

Microarray analysis of eosinophils reveals a number of candidate survival and apoptosis genes.

Geometric mean of the scaling (standard experiment) factor served as normalization factor/ 2 replicates

Hakonarson et al.³⁶

Oligonucleotide 5.000 genes

Rabbit and human ASM

Association between IL-1beta/TNF-alpha-induced glucocorticoid-sensitive changes in multiple gene expression and altered responsiveness in ASM.

Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs (matched-mismatched) / 2 replicates

Laprise et al.³⁷

Oligonucleotide 12.000 probe sets

8 subjects Lung tissue samples

Functional classes of bronchial mucosa genes that are differentially expressed in asthma.

Mean hybridization intensities of all probe sets on each array were scaled to a fixed level/ 2 replicates

Nakajima et al.³⁹

Oligonucleotide 12.000 genes

Human MCs and eosinophils

Gene expression screening of human mast cells and eosinophils using high-density oligonucleotide probe arrays: abundant expression of MBP in MCs

Mean hybridization intensities of all probe sets on each array were scaled to an arbitrary, fixed level / 1 replicate