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Table 2 Studies utilizing cDNA microarray technology to study asthma

From: Application of microarray technology in pulmonary diseases

Investigator

Microarray type

Species/Sample size

Summary/Key findings

Normalization procedure

 

Number of genes

Type of tissue

 

Replications per data point

Zou et al.32

cDNA 40.000 elements

10 monkeys Lung tissue samples

Microarray profile of differentially expressed genes in a monkey model of allergic asthma.

Ratios of Cy5/Cy3 multiplied to the balance coefficient of the microarray / 3 replicates

Brutsche et al.33

cDNA 600 genes

40 subjects Mononuclear cells

CAGE score for atopy and asthma.

Absolute difference of the expression of CAGE scored genes 1 replicate

Sayama et al.38

cDNA 14.000 genes

human umbilical cord mast cells

Transcriptional response of human mast cells stimulated via the Fc (epsilon) RI and identification of mast cells as a source of IL-11.

Array-specific normalization coefficient was calculated by centering in log base 2 space a dataset consisting of all elements with an I/D> 3-fold / 2 replicates

Brutsche et al.41

cDNA 600 genes

40 subjects Mononuclear cells

Apoptosis signals in atopy and asthma measured with cDNA arrays

G.I was normalized to the housekeeping G.I / 1 replicate

Syed et al. 42

cDNA 12.228 genes

Human CD4+ T cells

CCR7 down-regulation in asthma

Median G.I of each filter normalized any differences in cDNA probe activity between filters/ 1 replicate

Banerjee et al.43

cDNA 1.176 genes

18 mice Lung tissue samples

Gene expression profiling in inflammatory airway disease associated with elevated adenosine

G.I was normalized to the housekeeping G.I / 2 replicates

  1. Abbreviations: CAGE: Composite atopy gene expression, CCR7: Chemokine receptor 7, G.I: Gene Intensity, I/D: Intensity/Background ratio, Th: T helper, RI: Immunoglobulin receptor