Figure 7
EMSA illustrating DNA-binding activity of NF-κB to the NF-κB motif in lung tissue (A, C), and alveolar macrophages (B, D). The groups represent rats treated as follows: Saline, saline (IT); LPS, LPS (IT); LPS-SP600125, LPS (IT) and a pretreatment with SP600125 (IO), Saline-SP600125, saline (IT) and a pretreatment with SP600125 (IO); LPS-PD98059, LPS (IT) and a pretreatment with PD98059 (IO), Saline-PD98059, saline (IT) and a pretreatment with PD98059 (IO). Animals were sacrificed 4 hours after LPS treatment. Nuclear extracts were prepared in lung tissue and alveolar macrophages (5 × 106 alveolar macrophages). Addition of 100 ng of unlabeled cold competitor to the LPS samples successfully competed for NF-κB binding, and eliminated the specific band. Densitometry of NF-κB bands on EMSA is expressed in arbitrary densitometric units. Values are represented as means ± SEM of results from 5 rats in each group. * Significant differences compared with saline, p < 0.05, and + significant difference compared with LPS group, p < 0.05.