Figure 3
From: Protection of pulmonary epithelial cells from oxidative stress by hMYH adenine glycosylase
Western analysis of A549 cells over-expressing individual repair genes and effect on endogenous glycosylase level. (A) Endogenous expression of hOgg1 was not altered in A549 cells over-expressing any of the other repair genes when analyzed after non-toxic and toxic exposures. hOgg1 protein was not detectable for any of the cells under the above conditions when compared to cells over expressing hOgg1. (B) and (C) Endogenous expression of hMTH and hMYH respectively also were not altered in A549 cells over-expressing any of the other repair genes when analyzed after non-toxic and toxic exposures. (D) Endogenous expression of hNTH was analyzed under non-toxic and toxic conditions in A549 cells over-expressing the other repair genes. Reduced expression of hNTH was observed equally with all of the other genes after exposure to 95% O2. Endogenous expression of all four genes was equivalent under the above conditions in vector control cells; pSF91.1 (data not shown).