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Table 1 Summary of sensitivity and maximum responses to methacholine in airway and parenchymal lung compartments. Summary of the threshold dose (sensitivity) required to elicit a detectable increase in airway resistance (Raw), tissue damping (G) and tissue elastance (H) for naïve mice, mice systemically sensitised with OVA/Alum and challenged with PBS and mice systemically sensitised and challenged with OVA. Also shown is the percentage change in Raw, G and H in response to the maximum does of methacholine used (30 mg.mL-1). Data are presented as the mean (SEM).

From: The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose

Challenge Assessed after last aerosol (hr) Sensitivity - Threshold dose of MCh (mg.mL-1) Response at 30 mg.mL-1 MCh
   Raw G H Raw § G § H §
    p*   p*   p*   p*   p*   p*
Naïve - 0.54(0.14) - 0.51(0.25) - 0.10(0.02) - 235.5(21.5) - 138.8(6.1) - 145.4(5.1) -
1 PBS aerosol 24 and 48 pooled 0.55(0.30) ns 0.25(0.10) ns 0.06(0.01) ns 242.9(19.1) ns 141.5(4.7) ns 143.4(2.7) ns
1 OVA aerosol 24 0.09(0.03) 0.012 0.15(0.04) ns 0.05(0.01) ns 514.0(82.7) <0.05 275.5(30.6) <0.001 250.2(24.5) <0.001
  48 0.46(0.19) ns 0.18(0.05) ns 0.17(0.07) ns 326.4(32.6) ns 213.0(14.6) ns 179.3(12.6) ns
3 OVA aerosols 24 0.12(0.03) 0.012 0.35(0.20) ns 0.06(0.01) ns 271.2(43.1) ns 180.3(41.7) ns 178.9(39.9) ns
  48 0.20(0.07) 0.034 0.16(0.04) ns 0.08(0.01) ns 348.8(46.0) ns 243.6(42.7) ns 233.0(31.6) 0.045
6 OVA aerosols 24 0.17(0.05) 0.019 0.12(0.05) ns 0.06(0.01) ns 456.7(43.4) <0.05 358.2(88.4) <0.05 298.5(52.2) 0.02
  48 0.16(0.05) 0.034 0.15(0.06) ns 0.06(0.01) ns 396.0(23.3) <0.05 304.8(28.5) <0.05 295.6(30.6) 0.018
  1. § expressed as a % of saline response
  2. * vs naïve values