Figure 1
From: The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose
Timeline for sensitisation and data collection. Timeline for the protocols used to induce allergic bronchopulmonary inflammation and timing for bronchoalveolar lavage (BAL), serum IgE measurement and assessment of hyperresponsiveness to inhaled methacholine (MCh). Mice were systemically sensitised with two intraperitoneal injections of OVA/Alum on day 0 and 14, challenged with either 1 (A), 3 (B) or 6 (C) OVA aerosols (1%) for 30 minutes starting at day 21.