Aspergillusantigen induced similar increases in BAL fluid cell counts in wild-type,Mcp-1-/-andCcr2-/-mice. Total cells, macrophages, eosinophils, and lymphocytes are expressed as mean BAL fluid total cell counts ± SE from wild-type, Mcp-1-/- and Ccr2-/- mice (PBS-treated, N = 5 mice/group; Aspergillus antigen-treated, N = 8 mice/group; Aspergillus antigen exposure and sample collection are described in methods). Neutrophils represented <0.5% of total cells for all groups. The data shown are from one experiment and representative of three separate experiments. Asterisks (*) indicate values that are statistically significantly different (p < 0.001) compared to PBS controls.