Figure 5
From: A mechanism of airway injury in an epithelial model of mucociliary clearance
Representative zymograms from tissue (A) and mucus (B) shows the standards for MMP9 (top band, ~92 kD, latent size) and MMP2 (lower band, ~72 kD, latent size) in the leftmost lane. To the right of standard in each zymogram, two sets of bands are visible, corresponding to MMP-9 and MMP-2 levels of activity in duplicate samples of control tissue. In the next two lanes are duplicate sets of bands from an experiment which shows increased activated MMP-9 and possibly MMP-2 activity in sodium metabisulphite 10-1 M treated tissue in both tissue and mucus. The bar graph only shows a comparison of the scanning density of the MMP-9 bands since the MMP-2 control and sodium metabisulphite-treated tissue showed similar activation. A significant increase in activated MMP-9 was seen in sodium metabisulphite-treated mucus and tissue (* p < 0.05, n = 3 for each).