γδ T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-α and IFN-γ-producers in response to Pseudomonas aeruginosa

Table 2 Phenotypic characteristics of γδ T cells before and after 8 days of culture with cytosolic extract from P. aeruginosa (PA)

	Vγ9 day 0	Vγ9 day 8	Vδ2 day 0	Vδ2 day 8	CD4 day 0	CD4 day 8	CD8 day 0	CD8 day 8
Controls	84.71 ± 3.12**	92.85 ± 1.70	77.67 ± 3.8*	89.46 ± 3.43	2.03 ± 0.93	0.76 ± 0.65	11.92 ± 2.55	10.13 ± 1.91
CFw	89.83 ± 2.78*	94.75 ± 2.06	84.65 ± 3.89*	92.39 ± 3.23	0.49 ± 0.26	0.27 ± 0.18	9.21 ± 1.17	10.13 ± 1.91
CFp	92.52 ± 1.40**	96.82 ± 1.14	85.90 ± 3.88**	94.44 ± 2.72	0.78 ± 0.37	0.54 ± 0.31	9.48 ± 1.80	9.84 ± 2.69
CFn	82.80 ± 8.8	87.45 ± 7.98	79.46 ± 10.32	87.32 ± 8.64	0.1 ± 0.01	0.1 ± 0.01	8.41 ± 1.53	11.28 ± 3.42

Results are expressed as the percentage of positive cells (mean ± s.e.m.) in the γδ T subpopulation. CFw (cystic fibrosis patients, n = 14), CFp (cystic fibrosis patients infected by PA, n = 8), CFn (cystic fibrosis patients without PA, n = 4). Means comparison between paired samples (days 0 and 8) were made by the Student's t test: *Significantly lower than the value at day 8: p < 0.01 **Significantly lower than the value at day 8: p < 0.05

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