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Figure 6 | Respiratory Research

Figure 6

From: γδ T lymphocytes from cystic fibrosis patients and healthy donors are high TNF-α and IFN-γ-producers in response to Pseudomonas aeruginosa

Figure 6

Percentage of intracytoplasmic cytokine-producing αβ T cells (top) and γδ T cells (bottom) within total T lymphocytes after 10 days of culture with cytosolic extract fromPseudomonas aeruginosa, followed by 4 hs restimulation with PMA-Io. Intracytoplasmic cytokine expression was detected, by three color cytometry, in 10000 gated T lymphocytes. Column values correspond to mean, bar values correspond to standard error of the mean. Significant differences were not found between groups (ANOVA). p values were obtained from means comparison between αβ and γδ T lymphocytes within each group: controls (n = 17), Cystic fibrosis patients infected by PA (CFp, n = 9) and Cystic fibrosis patients not infected by PA (CFn, n = 4) (Student t test for paired samples).

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