Figure 10

Antibodies to PDGF (αPDGF) and TGF-β (αTGF) partially block platelet-induced stimulation of fibroblast-mediated collagen gel contraction. Collagen gels containing fibroblasts (HFL1) or fibroblasts and platelets (Plt) with or without antibodies were prepared. After gelation, the gels were released into media containing either antibodies to PDGF, TGF-β or the antibodies together. After 24 hours of culture the gel area was measured by an image analyser. Vertical axis: gel area expressed as percentage of initial gel area. Data presented as mean of triplicates ± SEM. ** P <0.01, *** P < 0.001.