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Figure 4 | Respiratory Research

Figure 4

From: Mass spectrometric analysis of electrophoretically separated allergens and proteases in grass pollen diffusates

Figure 4

SDS/PAGE and MADLI reflectron TOF of tryptic peptides derived from band 4 (Lolium perenne) and band 12 (Cynodon dactylon). A. Pollen diffusates (~ 20 μg: lane 1 Kentucky blue grass, lane 2 rye grass and lane 3 Bermuda grass) were separated using SDS/PAGE, stained with CBB and labelled bands excised, destained and digested with trypsin. B. Peptides from band 4 (rye grass) were analysed using MALDI reflectron TOF; digest peptides with masses corresponding to allergen Lol p 1 are labelled (*). C. Peptides from band 12 (Bermuda grass) were analysed using MALDI reflectron TOF, digest peptides with masses corresponding to allergen Cyn d 1 are labelled (*).

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Respiratory Research

ISSN: 1465-993X

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