Figure 2

SDS/PAGE and Western blot analysis of pollen diffusates. Approximately 10 μg of diffusate was loaded in each lane of a 12.5% SDS/PAGE and silver-stained (lanes 1–3). Lane 1, Kentucky blue grass (Poa pratensis); Lane 2, rye grass (Lolium perenne) and Lane 3, Bermuda grass (Cynodon dactylon): Lane 4, Kentucky blue grass; Lane 5, rye grass and Lane 6, Bermuda grass after Western blotting with a monoclonal antibody (IG 12) to allergen Phl p 1. MW markers are shown.