Table 1 List of antibodies used in this study
From: Allergic airway inflammation induces the migration of dendritic cells into airway sensory ganglia
Antibody | Source | Dilution |
|---|---|---|
Primary purified antibodies | ||
Rabbit polyclonal antibody against mouse, human PGP 9.5 | Abcam, Cambridge, UK | 1:200 |
Rat monoclonal antibody against mouse I-A/I-E | BioLegend, San Diego, USA | 1:200 |
Goat polyclonal antibody against mouse CGRP | Acris, Herford, Germany | 1:400 |
Armenian hamster monoclonal antibody against mouse CD11c | Abcam, Cambridge, UK | 1:100 |
Armenian hamster monoclonal antibody against mouse CD103 | eBioscience, San Diego, USA | 1:100 |
Rat monoclonal antibody against mouse CD11b | eBioscience, San Diego, USA | 1:100 |
Rat monoclonal antibody against mouse F4/80 | eBioscience, San Diego, USA | 1:100 |
Rabbit polyclonal antibody against mouse glutamine synthetase (GS) | Abcam, Cambridge, UK | 1:500 |
Rabbit Rabbit polyclonal antibody against Iba1 | Wako Chemicals, Japan | 1:300 |
Goat polyclonal antibody against GFAP | Abcam, Cambridge, UK | 1:200 |
Isotype control purified antibodies | ||
Rabbit IgG | Dianova, Hamburg, Germany | 1:5000 |
Rat IgG 2b kappa | BioLegend, San Diego, USA | 1:200 |
Goat IgG | R&D systems, | 1:300 |
Armenian Hamster IgG | BioLegend, San Diego, USA | 1:200 |
Secondary antibodies | ||
DyLight 488 conjugated Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |
Cy3 conjugated Donkey Anti-Rat IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:500 |
Cy3 conjugated Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |
DyLight 488 conjugated Donkey Anti-Goat IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |
Dylight 649 conjugated Goat Anti-Armenian Hamster IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |
DyLight 649 conjugated Donkey Anti-Rabbit IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |
Cy3 Conjugated Donkey Anti-Goat IgG | Jackson ImmunoResearch, INC., Baltimore, USA | 1:500 |
Cy5 conjugated Donkey Anti-Rat IgG antibody | Jackson ImmunoResearch, INC., Baltimore, USA | 1:400 |