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Figure 6 | Respiratory Research

Figure 6

From: Developmental lung expression and transcriptional regulation of Claudin-6 by TTF-1, Gata-6, and FoxA2

Figure 6

FoxA2 (A and B) and Gata-6 (C and D) induced Cldn6 transcription in bronchiolar Beas2B cells (A and C) and A-549 alveolar type II-like epithelial cells (B and D). FoxA2 significantly increased transcription in both cell types by acting on the 0.5-kb, 1.0-kb, and 2.0-kb proximal mouse Cldn6 promoters ligated into luciferase reporter vectors. Gata-6 did not significantly increase Cldn6 transcription in Beas2B cells transfected with the 0.5-kb construct (C); however, Gata-6 significantly increased transcription in Beas2B cells when the 1.0-kb and 2.0-kb constructs were available and in all reporter experiments performed in A-549 cells (D). Significant differences in luciferase activity normalized to β-galactosidase are noted at *P ≤ 0.05 when compared to non-transfected cells. The data shown are representative of experiments performed in triplicate.

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