Figure 5

TTF-1 induced Cldn6 transcription in bronchiolar Beas2B cells (A) and A-549 alveolar type II-like epithelial cells (B). TTF-1 induced transcription by acting on the 0.5-kb, 1.0-kb, and 2.0-kb proximal mouse Cldn6 promoters ligated into luciferase reporter vectors. In each case, TTF-1 significantly induced the transcription of the Cldn6 gene. Significant differences in luciferase activity, normalized to β-galactosidase used to assess transfection efficiency, are noted at *P ≤ 0.05 when compared to non TTF-1 transfected cells. The data shown are representative of experiments performed in triplicate.