Figure 1

Cldn6 mRNA Expression. Control C57Bl6 mice were screened by microarray analysis and Cldn6 expression levels were derived relative to GAPDH from E15-PN0 (A). Confirmatory quantitative RT-PCR was conducted using total RNA from embryonic C57Bl6 mice and results are presented relative to GAPDH (B). Representative data from experiments performed in triplicate are shown. *P ≤ 0.05 when comparisons were made between E15.5 and E16.5 or E15.5 and E17.5.