Figure 6From: Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levelsManganese and cadmium decrease the expression of CFTR in bronchial epithelial cells. 16HBE14o- cells were incubated with cadmium chloride (CdCl2) or manganese chloride (MnCl2) at the doses indicated for 24Â hours. CFTR protein was detected by immunobloting using a monoclonal antibody as described in Materials and Methods.Back to article page