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Table 4 miRNA expression changes in healthy ASM cells after treatment with FCS or Dex + FCS

From: Role of non-coding RNAs in maintaining primary airway smooth muscle cells

miRNA Baseline expression (ΔCT) FCS Dex + FCS
Microarray (FC) qRT-PCR (FC) Microarray (FC) qRT-PCR (FC)
hsa-miR-134 6.1 (<.001) - - −1.5 (<.001) −1.32 (<.05)
hsa-miR-150* 6.2 (<.001) - - −1.5 (<.001) −1.25 (<.05)
hsa-miR-371-5p 6.3 (<.001) −1.6 (<.001) −0.97 (<.05) −1.7 (<.001) −1.73 (<.05)
hsa-miR-718 5.4 (<.001) −1.5 (<.001) −1.26 (<.05) −1.6 (<.001) −1.44 (<.05)
hsa-miR-762 7.6 (<.001) −1.7 (<.001) - −1.9 (<.001) -
hsa-miR-940 6.7 (<.001) −1.5 (<.001) - −1.6 (<.001) -
hsa-miR-1181 7.0 (<.001) −1.7 (<.001) −0.89 (<.05) −1.8 (<.001) −0.89 (<.05)
hsa-miR-1207-5p 7.7 (<.001) −1.8 (<.001) −1.73 (<.05) −2.0 (<.001) −1.13 (<.05)
hsa-miR-1915 9.0 (<.001) −1.8 (<.001) −0.58 (<.05) −1.9 (<.001) −0.66 (<.05)
hsa-miR-3663-3p 8.0 (<.001) −1.8 (<.001) −1.10 (<.05) −2.0 (<.001) −1.36 (<.05)
hsa-miR-3665 9.7 (<.001) −2.1 (<.001) - −2.3 (<.001) -
hsa-miR-4271 6.1 (<.001) - - −1.5 (<.001) -
hsa-miR-4281 9.6 (<.001) −1.6 (<.001) - −1.5 (<.001) -
  1. This table lists those miRNAs that were differentially expressed in healthy ASM cells following stimulation with FCS (2.5%) or treatment with dexamethasone (10–7 M) before stimulation with FCS (2.5%). Baseline expression levels are expressed as Δ cycle threshold (ΔCT) and have been normalized against RNU44. Microarray data are denoted as the fold change against baseline control and is the mean of 9 individual patients. These values are for those genes that demonstrated an FDR of less than 0.05; associated P values are shown in parentheses. FC, Fold change.