Figure 4

Effect of dexamethasone and FCS upon the predicted mRNA targets of miRNAs decreased in expression in primary ASM cells. ASM cells were incubated with dexamethasone (10⁻⁷ M) for 1 h before being stimulated with FCS (2.5%) for 24 h. 9 mRNAs were changed in expression following treatment. To validate the array data, the expression of these mRNAs were confirmed by TaqMan RT-PCR. Bars represent mean ± SEM from 9 primary ASM cells. ^*/#p < 0.05 vs. baseline; ^**/##p < 0.01 vs. baseline; ^###p < 0.001 vs. baseline.