Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity

Respiratory Research

Table 1 Immunofluorescence against known phenotypic markers performed on fast and slow growing clonal populations

Phenotype markers	Slow growing clonal populations	Fast growing clonal populations
α-smooth muscle actin	++	+++
Fibroblast surface protein	++	+
Cathepsin K	+	++
Thromboxane synthase	++	++
α8 integrin	++	++

Staining intensity was categorized into 3 grades (+, low staining; ++, moderate staining; +++, intense staining) and recorded by blinded observations. Results represent observations from 3 independent studies.

ISSN: 1465-993X