Figure 6

Altered proliferative and signaling responses in human ASM clonal populations. In all panels each data point represents mean ± SEM (n = 3). Four Fast Growing clonal populations (•••) and four-five Slow Growing clonal populations (− − −) (as defined in the methods) were used in addition to the standard (not clonally derived) donor population (─). (A) [³H] thymidine incorporation in human ASM clonal populations in response to a range of PDGF-BB concentrations (0.00002 ng/ml to 20 ng/ml) or vehicle alone (Veh). Statistical analyses identifies significant difference in the proliferative response across all the clones (p < 0.001, two-way ANOVA). (B) cAMP formation in clonal human ASM populations in response to stimulation with varying concentrations of isoproterenol (10 μM to 1 nM) or vehicle alone (Veh). Significant differences were observed in cAMP formation across all the clonal populations (p < 0.001, two-way ANOVA). (C) [³H] Inositol Phosphate accumulation following stimulation of human ASM clonal populations with varying concentrations of bradykinin (10 μM to 0.1 nM) or vehicle alone (Veh). Statistical analyses of the data across all the clones showed a significant difference in the production of tritiated inositol phosphates (p < 0.001, two-way ANOVA). (D) [³H] Inositol Phosphate accumulation following stimulation of human ASM clonal populations with a range of histamine doses (100 μM to 1nM) or vehicle alone (Veh). Significant difference was observed in the total inositol phosphate accumulation across all the clonal groups following statistical analysis (p = 0.0023, two-way ANOVA).