Figure 4

Immunocytochemical localization of novel mesenchymal cell markers. Fast and slow growing clonally expanded human ASM cell populations were grown on 8-well chamber glass slides to 80% confluency. Following cell fixation, slow and fast growing clonal populations were immunocytochemically labelled for cathepsin K (A &C respectively; 2.5 μg/ml; B &D corresponding isotype control), thromboxane synthase 1 (E &G respectively; 8 μg/ml; F &H corresponding isotype control) and α8 integrin (I &K respectively; 1 μg/ml; J &L corresponding isotype control). Images were captured with ×20 objective lens using an epifluorescent microscope with matched exposure time and gains. Images are representative of 3 independent experiments.