Figure 3From: Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneityImmunocytochemical expression of standard mesenchymal cell marker. Fast and slow growing clonally expanded human ASM cell populations were grown on 8-well chamber glass slides to 80% confluency. Following cell fixation, slow and fast growing clonal populations were immunocytochemically labelled for α-smooth muscle actin (A &C respectively; 0.4 μg/ml; B &D corresponding isotype control) and fibroblast surface protein (E &G respectively; 0.4 μg/ml; F &H corresponding isotype control). Images were captured with ×20 objective lens using an epifluorescent microscope with matched exposure time and gains. Images are representative of 3 independent experiments.Back to article page