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Figure 3 | Respiratory Research

Figure 3

From: Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity

Figure 3

Immunocytochemical expression of standard mesenchymal cell marker. Fast and slow growing clonally expanded human ASM cell populations were grown on 8-well chamber glass slides to 80% confluency. Following cell fixation, slow and fast growing clonal populations were immunocytochemically labelled for α-smooth muscle actin (A &C respectively; 0.4 μg/ml; B &D corresponding isotype control) and fibroblast surface protein (E &G respectively; 0.4 μg/ml; F &H corresponding isotype control). Images were captured with ×20 objective lens using an epifluorescent microscope with matched exposure time and gains. Images are representative of 3 independent experiments.

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