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Figure 2 | Respiratory Research

Figure 2

From: Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-β1

Figure 2

Effect of TGF-β on glucocorticoid transrepression. (A) BEAS-2B cells were incubated with TGF-β (40pM) for 24 h, then incubated with dexamethasone (30 nM) for 30 min prior to stimulation with TNFα (10 ng/mL) for 4 h. RNA was then extracted and expression was analysed by qRT-PCR and expressed as fold change from control. (B) Data from (A) and (C) expressed as percentage inhibition by dexamethasone in the presence and absence of TGF-β. (C) A549 cells were incubated with TGF-β (40 pM) for 24 h, then incubated with dexamethasone (10 nM) for 30 min prior to stimulation with IL-1α (1 ng/mL) for 4 h (Gene expression) or 24 h (PGE2 determination). Gene expression was determined as in (A), PGE2 levels were determined by radioimmunoassay. Data are presented as mean and SEM for n = 3-4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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