Figure 1

Effect of TGF-β on glucocorticoid transactivation. BEAS-2B cells were incubated with TGF-β (4-100pM) for 24 h before stimulation by dexamethasone (1-100 nM). (A) GRE activity was measured in BEAS-2B cells transiently transfected with a GRE-SEAP reporter construct, incubated with TGF-β (4-100 pM), then stimulated with dexamethasone for a further 24 hours. The level of SEAP in the supernatants was expressed as a percentage the level induced in response to 30nM dexamethasone. (B) Glucocorticoid-inducible gene expression in non-transfected cells. BEAS-2B cells were incubated with TGF-β (40 pM) for 24 h before stimulation by dexamethasone (30 nM) for 4 h after which RNA was extracted and analysed by qRT-PCR. Gene expression is expressed as fold change from control. Data are presented as mean and SEM for n = 4 independent experiments. *P < 0.05, **P < 0.01, *P < 0.001 c.f. Control (A), 30 nM Dex (B).