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Figure 4 | Respiratory Research

Figure 4

From: Arsenic trioxide inhibits transforming growth factor-β1-induced fibroblast to myofibroblast differentiation in vitro and bleomycin induced lung fibrosis in vivo

Figure 4

TGF-β1 induced PAI-1, fibronectin and Smad3 phosphorylation are impaired in PML -/- MEFs. (A) NHLFs were treated with ATO (20nM) for 24 hrs. Cytoplasmic and nuclear compartments were separated and extracted. PML protein expression was decreased in both the cytoplasm and nucleus in response to ATO treatment. (B) Immunofluorescent staining shows that PML bodies in NHLFs were decreased in response to ATO treatment. (C) Wild type and PML-/- mouse embryonic fibroblasts (MEFs) were treated with TGF-β1 (1 ng/ml) for 24 hrs. Fibronectin-EDA and PAI-1 protein expression was induced by TGF-β1 in WT-MEFs but not PML -/- MEFs. (D) Wild-type and PML -/- MEFs were treated with TGF-β1 (1 ng/ml) and cells were harvested at the indicated time points. Smad3 phosphorylation was induced in WT-MEFs and the level of phosphorylation was markedly decreased in PML -/- MEFs. Western blot data represents consistent trend in three independent repeats with the best image quality.

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Respiratory Research

ISSN: 1465-993X

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