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Figure 1 | Respiratory Research

Figure 1

From: Arsenic trioxide inhibits transforming growth factor-β1-induced fibroblast to myofibroblast differentiation in vitro and bleomycin induced lung fibrosis in vivo

Figure 1

ATO inhibits TGF-β1-induced α-SMA and collagen expression. (A-D) Normal human lung fibroblasts (NHLFs) were serum starved overnight and treated with Arsenic trioxide (ATO) for 24 hrs, then treated with TGF-β1 (1 ng/ml) for 24 hrs. α-SMA, Collagen-1, PAI-1 and CTGF mRNA expression levels were evaluated by TGF-β1 and ATO inhibited TGF-β1’s effect. (E) TGF-β1-induced α-SMA and collagen-1 protein expression was blocked by ATO in NHLFs. (F) Immunofluorescent staining showed TGF-β1-induced α-SMA fiber formation was reduced by ATO. (G) An MTT assay showing that ATO does not affect NHLF viability. RT-PCR Data represent results from three independent experiments with duplicate repeats. Western blot and immunofluoresent staining data represent consistent trend in three independent repeats with the best image quality. *P value < 0.05, **P value <0.01, ***P value < 0.001.

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