Bifidobacterium breve and Lactobacillus rhamnosus treatment is as effective as budesonide at reducing inflammation in a murine model for chronic asthma

Table 3 Overview of the hitstological and Immunohistochemical score

Group	Inflammation (HE)	Goblet cells (PAS)	Connective tissue (Masson)	α-smooth muscle cells (anti-α-smooth muscle actin)	Proliferating cells (anti-Ki67)
OVA/Sal-PBS	-	-	-	+	-
OVA/OVA-PBS	++	+	++	++	++
OVA/Sal-B. breve	-	-	+	+	+/-
OVA/OVA-B. breve	+	+	+	++	+
OVA/Sal-L. rhamnosus	-	-	+	+	+/-
OVA/OVA-L. rhamnosus	+	+	+	++	+/-
OVA/Sal-budesonide	-	-	+	+	+/-
OVA/OVA-budesonide	+	-	+	++	+

Slides were reviewed in blinded fashion by two observers independently and slides were scored on the basis of the percentage of positive stained cells in the following way: -,no positive staining; +/-, less than 25% of cells stained positive; +,25 to 50% cells stained positive; ++, 50 to 75% cells stained positive. Results are presented as average score of 5 animals/group.

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