Figure 5

A) Pax7, B) MyoD, C) Myf5, D) Myogenin, E) MRF4 and F) Numb protein accumulation during myogenesis in satellite cells isolated and cultured from healthy subjects (white bars; n = 7) and patients with COPD (black bars; n = 8). Protein extraction was performed daily, the first three days represent the myoblasts phase past than is the differentiation phase (day 3 to day 9). Signal analysis was calculated by densitometry and reported over a positive control. When a is present: statistically significant difference from control (ANOVA; p < 0.05).