Figure 2From: Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary diseaseCentral nuclei detection performed by immunostaining. (A) Cryosections were analyzed by immunostaining for nuclei (blue) and laminin (red). Immuno-detection is shown for 10 μm skeletal muscle cryosections. The number of central nuclei (white arrows) is expressed over 100 fibers and reported as a ratio. Ratios are calculated from 9 healthy subjects, 11 COPD (MTCSA > 70 cm2) and 6 COPD (MTCSA < 70 cm2). (B) Nucleus inside the perimeter of a given fiber delimited by laminin was counted as a central nucleus, expressed over 100 fibers and reported as a ratio. When a is present: statistically significant difference from control. When b is present b: statistically significant difference from COPD > 70 cm2 (ANOVA; p < 0.05).Back to article page