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Figure 2 | Respiratory Research

Figure 2

From: Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease

Figure 2

Central nuclei detection performed by immunostaining. (A) Cryosections were analyzed by immunostaining for nuclei (blue) and laminin (red). Immuno-detection is shown for 10 μm skeletal muscle cryosections. The number of central nuclei (white arrows) is expressed over 100 fibers and reported as a ratio. Ratios are calculated from 9 healthy subjects, 11 COPD (MTCSA > 70 cm2) and 6 COPD (MTCSA < 70 cm2). (B) Nucleus inside the perimeter of a given fiber delimited by laminin was counted as a central nucleus, expressed over 100 fibers and reported as a ratio. When a is present: statistically significant difference from control. When b is present b: statistically significant difference from COPD > 70 cm2 (ANOVA; p < 0.05).

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