Figure 5
From: JNK suppresses pulmonary fibroblast elastogenesis during alveolar development
JNK suppresses pulmonary fibroblast elastogenesis in vitro . (A) JNK1−/− and JNK2%3−/− PND14 lung fibroblasts contained more tropoelastin mRNA than wild type lung fibroblasts. (B) The media from JNK-deficient lung fibroblasts trended towards having more soluble tropoelastin than media from wild type lung fibroblasts. (C) Infection of PND14 JNK2%3−/− lung fibroblasts (which also contained floxed JNK1 alleles) with cre-expressing adenovirus (Ad-Cre) was effective at reducing JNK1 mRNA levels when compared to fibroblasts infected with a green fluorescent protein adenovirus (Ad-GFP). (D) Deletion of all three JNK isoforms from pulmonary fibroblasts resulted in increased tropoelastin mRNA compared to deletion of only JNK2%3. E-The JNK activator anisomycin reduced wild type PND14 lung fibroblast tropoelastin mRNA in a dose-dependent manner. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA. All analyses were performed in triplicate.