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Figure 5 | Respiratory Research

Figure 5

From: Regulation of transplanted mesenchymal stem cells by the lung progenitor niche in rats with chronic obstructive pulmonary disease

Figure 5

SDF-1 is upregulated by HIF-1α under hypoxia. First, we investigated changes in the expression of HIF-1α and SDF-1 under hypoxic conditions. The expression of HIF-1α and SDF-1 mRNAs increased significantly in A549 cells under hypoxic conditions compared with that under normoxic conditions (C, D). Next, we investigated the effects of transfecting A549 cells with siRNA against HIF-1α. Control siRNA was labeled with red fluorescence. Using Lipofectamine™ 2000, an 80% siRNA transfection efficiency was achieved at approximately 6 h (A, B). The expression of HIF-1α and SDF-1 mRNAs was decreased significantly in A549 cells transfected with siRNA against HIF-1α compared with that in A549 cells transfected with control siRNA (E, F). Western blotting showed that the expression level of HIF-1α protein was also decreased significantly in A549 cells transfected with siRNA against HIF-1α (G, H). ELISA results demonstrated that the level of SDF-1 was decreased significantly in the culture medium of A549 cells transfected with siRNA against HIF-1α (I). The results are expressed as the mean ± SD, n = 10 in each group (*P < 0.05).

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