Figure 5

Vegf165 attenuation of lung growth retardation requires NRP1. E11.5 + 2 lung explants were cultured for 2 d. under the indicated conditions and total number of terminal branches and total branch length quantitated. (A) 50% oxygen alone (B) 50% oxygen + 100 ng/ml of Vegf165 (C) 50% oxygen + isotype control antibody for 30 minutes and then 100 ng/ml of Vegf165 (D) 50% oxygen + anti neuropilin-1 antibody (anti-NRP1, 10 microgram/ml) for 30 minutes and then 100 ng/ml of Vegf165 (E) total number of terminal branches and (F) total branch length from lungs cultured as above. n = 4, *p <0.05 compared to 50% oxygen + Vegf165. Photographs at 4× magnification. Bar = 1 mm.