Figure 1

Ad-Vegf188 rescues hyperoxia suppression of Vegf188 mRNA expression. A’) Adenoviral control infection (Ad-DsRed) in mouse explanted embryonic lung. (A) E12.5 explanted lung infected with adenovirus (Hoffmann image). (B) Same lung under immunofluorescence. Photographs at 10x magnification. a: airway, m: mesenchyme. B’) E10.5 lung explants were harvested, infected with either Ad-DsRed control adenovirus or Ad-Vegf188 and then exposed at E12 to either 3% or 50% oxygen for 2 d. Quantitative PCR of Vegf188 was performed with Gapdh used as control. n = 4, *p <0.05 vs. 3% alone and 50% oxygen + AdVegf188 at E12 + 2.