Figure 6

RT-PCR and Western blotting analyses. (A) RT-PCR for PAR-1 and -2 and (B) Western blotting for PAR-2 in hTERT-transfected HNECs after treatment with 0.1 U Pseudomonas aeruginosa elastase. (C) RT-PCR and (D) Western blotting for PAR-2, occludin, and claudin-1 in hTERT-transfected HNECs after treatment with siRNA of PAR-2 for 48 h. (E) Western blotting for PAR-2, occludin, and claudin-1 in hTERT-transfected HNECs pretreated with PAR-2 siRNA before treatment with 0.1 U Pseudomonas aeruginosa elastase for 1 h. The corresponding expression levels are shown as bar graphs. PE: Pseudomonas aeruginosa elastase.