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Figure 1 | Respiratory Research

Figure 1

From: Impact of heterozygote CFTR Mutations in COPD patients with Chronic Bronchitis

Figure 1

Effect of CFTR mutation heterozygosity on smoke-induced CFTR dysfunction in vitro . Well differentiated primary human bronchial epithelial cells (HBE) cells were isolated from healthy non-smokers and non-CF individuals and expressing either wild type CFTR (CFTR +/+) or heterozygous F508del CFTR mutation (CFTR +/-). These cells were exposed to cigarette smoke generated from 1 cigarette for 10 min and CFTR function measured in Ussing chambers under voltage clamp conditions. A) Representative tracings of forskolin stimulated CFTR ion transport in HBE cells in vitro. Addition of forskolin (20 μM) and CFTRinh172 (10 μM) are indicated. B) Forskolin-stimulated CFTR-dependent anion transport is summarized, N = 6-8 *P < 0.05, **P < 0.005.

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