Effect of pirfenidone on macrophages in bronchoalveolar lavage (BAL) fluid. BAL fluid was collected from saline- or bleomycin (BLM)-treated mice with or without pirfenidone administration on day 14. Cell count in BAL fluid for macrophages is shown. The number of macrophages was increased in BLM-treated mice compared with that in saline-treated mice, and pirfenidone attenuated this increase. Data are presented as means ± standard error from 2 separate experiments. n = 5 in each group. PFD, pirfenidone.