Table 1 Primers used for real-time quantitative polymerase chain reaction in rattus norvegicus thoracic aorta and pulmonary arteries
From: Pulmonary vasoreactivity in spontaneously hypertensive rats - Effects of endothelin-1 and leptin
Genes | Sequences | |
|---|---|---|
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) | Sense | 5′ – ACAGGCCAGACTTTGTTGGA – 3′ |
Antisense | 5′ – TCCACTTTCGCTGATGACAC – 3′ | |
Endothelin receptor type A (ETA) | Sense | 5′ – CTGGTGGCTCTTTGGATTCT – 3′ |
Antisense | 5′ – GCTCCCATTCCTTCTGTTGA – 3′ | |
Endothelin receptor type B (ETB) | Sense | 5′ – CGATTGTATCATGCCTCGTG – 3′ |
Antisense | 5′ – GGGACCATTTCTCATGCACT – 3′ | |
Leptin | Sense | 5′ – CCTGTGGCTTTGGTCCTATC – 3′ |
Antisense | 5′ – ATACCGACTGCGTGTGTGAA – 3′ | |
Leptin receptor | Sense | 5′ – GGACAGCCAAACAAAAGCAC – 3′ |
Antisense | 5′ – TATCCGAGACGATTTCAGCA – 3′ | |
Endothelial nitric oxide synthase (eNOS) | Sense | 5′ – GGTATTTGATGCTCGGGACT – 3′ |
Antisense | 5′ – TGATGGCTGAACGAAGATTG – 3′ | |
Inducible nitric oxide synthase (iNOS) | Sense | 5′ – GTTTCCCCCAGTTCCTCACT – 3′ |
Antisense | 5′ – CTCTCCATTGCCCCAGTTT –5′ | |
Preproendothelin-1 (PPET-1) | Sense | 5′ – CAGACCAAGGGAACAGATGC – 3′ |
Antisense | 5′ – ACGCCTTTCTGCATGGTACT – 3′ | |
Endothelin-converting enzyme 1 (ECE1) | Sense | 5′ – GCCCACCAAGAACGAGATT – 3′ |
Antisense | 5′ – GACCCCGATACCAAAGT – 3′ | |
Suppressor of cytokine signaling 3 (SOCS3) | Sense | 5′ – TGCAGGAGAGCGGATTCTAC – 3′ |
Antisense | 5′ – AGCTGTCGCCCATAAGAAAG – 3′ |